Quantitative Determination of Gallotannin in Paeoniae Radix

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Abstract
Gallotannins (tetra-undecagalloylglucose) in Paeoniae Radix were separated according to the degree of galloylation (number of galloyl groups per glucose) by normal phase high performance liquid chromatography and quantitatively determined. Total gallotannin contents of Paeoniae Radix produced in Hokkaido, Nara prefecture [Japan] and China were 0.52-1.16%, and there was no difference among 3 groups of samples. The average degree of galloylation was calculated from the contents of tetra-undecagalloylglucose, and those of Paeoneae Radix produced in Hokkaido (5.88-7.36) appeared to be higher than those in Nara prefecture (5.30-5.72) and China (5.10-5.75). The total gallotannin contents and the average degree of galloylation varied according to the preparation methods of Paeoniae Radix, and the total gallotannin contents in the aerial parts of peony were 5-15 times higher than that of root on a fresh weight basis. In the decoction of 2 Kanpo precriptions, Shakuyaku-kanzo-to and Keishi-ka-shakuyaku-to, only tetra and pentagalloylglucoses were detected as gallotannin.