Reconstitution of chromatin core particles
- 1 November 1977
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 16 (24) , 5295-5303
- https://doi.org/10.1021/bi00643a021
Abstract
Chromatin core particles [chicken blood cell nuclei] containing 140 base pairs (bp) of DNA plus the inner histones, can be nearly quantitatively formed either by reassociation from 2 M NaCl or by reconstitution from salt extracted histones and DNA. The reassociated or reconstituted particles appear to be identical with the native particles in all physical properties examined (sedimentation velocity, histone content, circular dichroism, and melting) as well as in their patterns of digestion by micrococcal nuclease, DNase I and trypsin. In the presence of excess DNA, no half-particles are formed. In the presence of excess histone, aggregated structures are formed in addition to 11S core particles.This publication has 20 references indexed in Scilit:
- Ribosomal Genes of Xenopus laevis : Evidence of Nucleosomes in Transcriptionally Active ChromatinScience, 1976
- A possible explanation for the nuclease limit digestion pattern of chromatin.Proceedings of the National Academy of Sciences, 1976
- Chromosomal Subunits in Active Genes Have an Altered ConformationScience, 1976
- Chromatin structure as probed by nucleases and proteases: Evidence for the central role of hitones H3 and H4Cell, 1976
- Nucleosome structure in Aspergillus nidulansCell, 1976
- Differences and similarities in chromatin structure of neurospora crassa and higher eucaryotesCell, 1976
- The organization of histones and DNA in chromatin: Evidence for an arginine-rich histone kernelCell, 1976
- Physical Studies of ChromatinEuropean Journal of Biochemistry, 1976
- The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresisBiochemical Journal, 1967
- Temperature measurement and control of analytical rotors in the ultracentrifugeAnalytical Biochemistry, 1965