Nuclear magnetic resonance description of molecular motion and phase separations of cholesterol in lecithin dispersions.
- 1 November 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (11) , 3812-3815
- https://doi.org/10.1073/pnas.73.11.3812
Abstract
Proton-enhanced 13C NMR is used to obtain signals from labeled cholesterols in lecithin dispersions. The [26-13C]cholesterol resonance indicates that the aliphatic tail of the molecule undergoes reorientation fast enough to average completely the chemical shift anisotropy. In contrast, [4,13C]cholesterol signals are characteristic of limited anisotropic reorientation. The resonances from the 4 position are sensitive to the temperature-concentration phase diagram. A phase boundary is observed at about 20 mol % cholesterol.This publication has 17 references indexed in Scilit:
- Interactions of proteins and cholesterol with lipids in bilayer membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturingBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- APPLICATION OF PROTON-ENHANCED NUCLEAR INDUCTION SPECTROSCOPY TO THE STUDY OF MEMBRANESAnnals of the New York Academy of Sciences, 1973
- Lateral phase separations in binary mixtures of cholesterol and phospholipidsBiochemical and Biophysical Research Communications, 1973
- Phase transitions of phospholipid bilayers and membranes of Acholeplasma laidlawii B visualized by freeze fracturing electron microscopyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- Nuclear magnetic resonance study of lecithin-cholesterol interactionsJournal of Molecular Biology, 1972
- Deuteron resonance: A novel approach to the study of hydrocarbon chain mobility in membrane systemsFEBS Letters, 1971
- Effects of cholesterol and cholesterol derivatives on hydrocarbon chain mobility in lipidsBiochemical and Biophysical Research Communications, 1971
- Molecular motion in spin-labeled phospholipids and membranesJournal of the American Chemical Society, 1971
- Nuclear Magnetic Resonance Spectroscopic Studies of the Interaction of Phospholipids with CholesterolNature, 1966