Reproducibility of vitamin assays with mutants of Neurospora crassa maintained by freeze-drying

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Abstract
Three mutant strains (ATCC 9277, ATCC 9278, ATCC 9683) of Neurospora crassa requiring choline, inositol, and p-aminobenzoic acid respectively for normal growth were deposited at the ATCC by G. W. Beadle 30 years ago and were preserved at various time intervals by freeze-drying. Each preservation batch yielded cultures that, when used for biological assays, exhibited the same biochemical properties as they originally possessed. The freeze-drying technique is shown to be applicable to preserving biological properties of sporulating fungi for bioassay over prolonged periods of time.

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