Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2
- 19 December 2003
- journal article
- Published by Wiley in Environmental Microbiology
- Vol. 6 (3) , 191-197
- https://doi.org/10.1046/j.1462-2920.2003.00550.x
Abstract
Alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented. To identify the genes responsible for alkane degradation in this organism, two putative genes for alkane hydroxylases were cloned from Alcanivorax borkumensis SK2. They were named alkB1 and alkB2. These genes were subsequently disrupted in A. borkumensis SK2, and the growth phenotypes of the disruptants were examined. The results indicate that the alkB1 gene is responsible for the degradation of short-chain n-alkanes. A double mutant defective in both alkB1 and alkB2 was still able to grow on medium-chain n-alkanes, indicating that genes other than alkB1 and alkB2 are also involved in n-alkane hydroxylation by A. borkumensis SK2.Keywords
This publication has 20 references indexed in Scilit:
- Differential Expression of the Components of the Two Alkane Hydroxylases from Pseudomonas aeruginosaJournal of Bacteriology, 2003
- Gene Cloning and Characterization of Multiple Alkane Hydroxylase Systems inRhodococcusStrains Q15 and NRRL B-16531Applied and Environmental Microbiology, 2002
- Robust Hydrocarbon Degradation and Dynamics of Bacterial Communities during Nutrient-Enhanced Oil Spill BioremediationApplied and Environmental Microbiology, 2002
- Functional Analysis of Alkane Hydroxylases from Gram-Negative and Gram-Positive BacteriaJournal of Bacteriology, 2002
- Biodegradation of n -Alkylcycloalkanes and n -Alkylbenzenes via New Pathways in Alcanivorax sp. Strain MBIC 4326Applied and Environmental Microbiology, 2001
- Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacteriumInternational Journal of Systematic and Evolutionary Microbiology, 1998
- Cycloclasticus pugetii gen. nov., sp. nov., an Aromatic Hydrocarbon-Degrading Bacterium from Marine SedimentsInternational Journal of Systematic and Evolutionary Microbiology, 1995
- Genetics of alkane oxidation byPseudomonas oleovoransBiodegradation, 1994
- Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in transProceedings of the National Academy of Sciences, 1979
- Subterminal Oxidation of Aliphatic Hydrocarbons by MicroorganismsCRC Critical Reviews in Microbiology, 1971