Comparative rates of transfer of lipid-linked oligosaccharides to endogenous glycoprotein acceptors in vitro.

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Abstract
Particulate preparations of Nil 8 fibroblasts catalyze the synthesis of 2 oligosaccharide-lipids: 1 composed of N-acetylglucosamine and mannose residues and the other containing, in addition to mannose and N-acetylglucosamine, 1 or 2 glucose residues. These 2 oligosaccharide-lipids were purified and added to fresh microsomal preparations. In comparative studies, the glucose-containing lipid-linked oligosaccharide is transferred much more rapidly to endogenous protein acceptors than the glucose-free compound. With materials of comparable specific activities, as much as 41% of the glucose-containing oligosaccharide was transferred to protein as compared to 5% for the glucose-free compound. The attachment of glucose to mannosyl lipid-linked oligosaccharide serves an important role in the transfer of these compounds from lipid carrier to protein acceptor.