Liver Storage of Vitamin B12 by the Rat.

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Abstract
Liver homogenates from rats fed graded levels of crystalline vit. B12 or of crude supplements were treated for vit. B12 potency with Lactobacillus leichmannii after short-term autoclaving at pH 7. The vit. B12 potency increased as the level fed was increased. The values were only 5-10% of those for beef liver and were not reduced appreciably by alkali treatment. Studies of the desoxyribonucleic acid levels in rat livers and assays with Euglena gracilis indicated that the residual vit. B12 activity, as determined with L. leichmannii, may have been due to the presence of alkali stable forms of vit. B12, as well as to desoxyribosides. The liver weight was greater per unit of body wt. for the rats fed the vit. Bl2-deficient diet than for those fed the optimum diet.

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