Standardized culture methods for bryophyte plants, tissues, and propagules were developed. The methods overcome many past inconveniences and offer certain advantages that justify a broader use of bryophyte material for fundamental physiological and biochemical research. Five substrates prove to be especially advantageous for the culture of the liverwort, Marchantia polymorpha. They include vermiculite, perlite, glass cloth, nutrient agar, and nutrient solution. Selected culture conditions and vessels especially adapted to each substrate are described. The methods are contrasted for ease of culture maintenance, yields, and amenability to specific experimentation.