Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium

Abstract
The nucleotide sequence of the entire histidine transport operon from S. typhimurium was determined and is shown to consist of 4 genes, hisJ, hisO, hisM and hisP. This operon provides the only example of a binding protein-dependent transport system for which the total number of protein component is known. Determination of the amino acid compositions and sequences of these 4 transport proteins, together with analysis of various transport mutants, allows the proposal of a molecular model for binding protein-dependent transport.