Cloning and expression of the putative gene coding for GTP cyclohydrolase I from Escherichia coli
- 1 January 1990
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 66 (1-3) , 231-234
- https://doi.org/10.1016/0378-1097(90)90288-2
Abstract
The putative gene coding for GTP cyclohydrolase I of Escherichia coli was isolated from a λgt11 expression vectori library by using antibodies as a probe and has been subcloned on a 3.8 kb BamHI fragment in the plasmid vector pUC13. E. coli cells carrying the recombinant plasmid designated pCYH express 100-fold increased levels of the enzyme. The protein formed under the control of the plasmid appears electrophoretically and immunochemically identical with the wild type enzyme.Keywords
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