The nucleotide sequences of the initiator transfer RNAs from bean cytoplasm and chloroplasts

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Abstract
The initiator tRNAsMet from the cytoplasm and chloroplasts of Phaseolus vulgaris have been purified and sequenced. The sequence of bean oytoplasmic initiatortRNAsiMet is : pA-U-C-A-G-A-G-U-m1G-m2G-C-G-C-A-G-C-G-G-A-A-G-C-G-U-m2G-G-V-G-G-G-C-C-C-A-V-t6A-A-C-C-C-A-C-A-G-m7G-D-m5C-C-C-A-G-G-A-ψ-C-G-m1-A-A-C-C-U-Gm-G-C-U-C-U-G-A-U-A-C-C-AOH. The sequence of bean cytoplasmic tRNAiMet is almost identical to that of wheat germ and shows a high degree of homology with other cytoplasmic initiator tRNAs. The sequence of bean chloroplast initiator tRSAfMet is : pC-G-C-G-G-A-G-U-A-G-A-G-C-A-A-C-U-U-Gm-G-D-A-G-C-V-C-G-C-A-A-G-G-C-U-C-A-U-A-A-C-C-U-U-G-A-A-m7G-acp3U-U-A-C-G-G-G-T-ψ-C-A-A-A-U-C-C-C-G-U-C-U-C-C-G-C-A-A-C-C-AOH. Bean chloroplast initiator tRSAfMet sequence shows procaryotic characteristics at the 5′ end of the acceptor stem and in the TψC loop, but also contains some distinctive features.