Sugar transport in Trypanosoma brucei: a suitable kinetic probe
- 1 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 194 (1) , 126-130
- https://doi.org/10.1016/0014-5793(86)80063-1
Abstract
A transport assay has been developed for use in the investigation of 1-deoxy-D-glucose uptake in trypanosomes. 1-Deoxy-D-glucose has high affinity for the trypanosome sugar transport system (net influx K m = 4.03 ± 0.42 mM; V = 0.052 ± 0.005 mM·s−. D-Glucose oxidation is competitively inhibited by 1-deoxy-Dglucose. However, we show that 1-deoxy-D-glucose is not a substrate for metabolism and that the competition occurs because of interaction at the transport system. D-Glucose competitively inhibits 1-deoxy-D-glucose influx.Keywords
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