Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast
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- 25 September 2009
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 325 (5948) , 1693-1696
- https://doi.org/10.1126/science.1173759
Abstract
We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.Keywords
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