Pyrimidine Nucleotides Do Not Affect the Enzymes of Glutathione Biosynthesis

1 July 1985

journal article
research article
Published by S. Karger AG in Enzyme

Vol. 34 (2) , 94-97
https://doi.org/10.1159/000469368

Abstract

Erythrocytes from patients with hereditary pyrimidine 5'-nucleotidase (P5N, EC 3.1.3.5) deficiency accumulate large quantities of several pyrimidine nucleotides and their derivatives. In addition, the reduced glutathione (GSH) concentration is elevated in erythrocytes from patients with this enzyme deficiency. In the present study, we were unable to demonstrate any effect of various pyrimidine nucleotides and their derivatives on enzymes of glutathione biosynthesis and metabolism. Thus, elevation of GSH levels in erythrocytes from P5N patients is not a result of modulation of these enzymes by pyrimidine nucleotides and their derivatives.

Keywords

This publication has 9 references indexed in Scilit:

Hemolytic anemia in hereditary pyrimidine 5′-nucleotidase deficiency. II. Effect of pyrimidine nucleotides and their derivatives on glycolytic and pentose phosphate shunt enzyme activity
Clinica Chimica Acta; International Journal of Clinical Chemistry, 1984
IDENTIFICATION OF CYTIDINE DIPHOSPHODIESTERS IN ERYTHROCYTES FROM A PATIENT WITH PYRIMIDINE NUCLEOTIDASE DEFICIENCY
1984
Erythrocyte nucleotide pattern in two children in a Norwegian family with pyrimidine 5'-nucleotidase deficiency
Clinica Chimica Acta; International Journal of Clinical Chemistry, 1983
HEMOLYTIC-ANEMIA IN HEREDITARY PYRIMIDINE 5'-NUCLEOTIDASE DEFICIENCY - NUCLEOTIDE INHIBITION OF G6PD AND THE PENTOSE-PHOSPHATE SHUNT
1982
Glutathione transport by inside-out vesicles from human erythrocytes.
Proceedings of the National Academy of Sciences, 1980
Distribution of Erythrocyte Nucleotides in Pyrimidine 5'-Nucleotidase Deficiency
British Journal of Haematology, 1979
Glutathione synthetase. Purification from rat kidney and mapping of the substrate binding sites.
Journal of Biological Chemistry, 1979
gamma-Glutamylcysteine synthetase. Further purification, "half of the sites" reactivity, subunits, and specificity.
Journal of Biological Chemistry, 1977
Human erythrocyte pyrimidine nucleoside monophosphate kinase. Partial purification and properties of two allelic gene products.
Journal of Biological Chemistry, 1976