Evidence forin vivo upregulation of 1,25(OH)2 vitamin D3 receptor in human monocytes
- 30 June 1989
- journal article
- research article
- Published by Springer Nature in Calcified Tissue International
- Vol. 45 (4) , 255-256
- https://doi.org/10.1007/bf02556046
Abstract
Mammalian cells increase net expression of 1,25(OH)2D3 receptors after exposure to physiological concentrations of 1,25(OH)2D3 in vitro. we examined specific binding of 1,25(OH)2D3 by human monocytes before and after daily administration of 1.5–2 ug 1,25(OH)2D3 p.o. for 3 days in 5 healthy normal D-replete probands. Median specific binding (Nmax) at baseline was 793 molecules/cell and 2052 or 2828 at 24h and 72h of 1,25(OH)2D3 treatment respectively. The results suggest (a) up-regulation of 1,25(OH)2D3 receptors occurs in man and (b) monocyte preparations can be used to assess receptor regulationin vivo.Keywords
This publication has 8 references indexed in Scilit:
- Evidence for in vivo upregulation of the intestinal vitamin D receptor during dietary calcium restriction in the rat.Journal of Clinical Investigation, 1988
- Reduced Binding of [3H]l,25-Dihydroxyvitamin D3in the Parathyroid Glands of Patients with Renal FailureNew England Journal of Medicine, 1987
- Measurement of 1,25-Dihydroxyvitamin D3 Receptor Turnover by Dense Amino Acid Labeling: Changes during Receptor Up-Regulation by Vitamin D Metabolites*Endocrinology, 1987
- Homologous up-regulation of the 1,25 (OH)2 vitamin D3 receptor in ratsBiochemical and Biophysical Research Communications, 1986
- No 1,25-dihydroxyvitamin D3 receptors on osteoclasts of calcium-deficient chicken despite demonstrable receptors on circulating monocytes.Journal of Clinical Investigation, 1986
- Demonstration and characterization of 1,25-dihydroxyvitamin D3 receptors in human mononuclear blood cellsBiochemical and Biophysical Research Communications, 1984
- Antigens on human monocytes identified by monoclonal antibodies.The Journal of Immunology, 1981
- Enhancement of sheep red blood cell human lymphocyte rosette formation by the sulfhydryl compound 2-amino ethylisothiouronium bromideClinical Immunology and Immunopathology, 1975