Insulin stimulates erythroid colony formation independently of erythropoietin*
- 1 February 1983
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 53 (2) , 311-316
- https://doi.org/10.1111/j.1365-2141.1983.tb02025.x
Abstract
Summary. The effect of insulin on the proliferation of late stage erythroid precursor cells (CFU‐e) from fetal mouse liver and aduit bone marrow was studied in a serum‐free culture system. Insulin in supraphysiological concentrations (> 10 −8m) stimulated erythroid colony formation independently of Ep. The combined effect of Ep and insulin was smaller than the surn of their single effects. The number of colonies obtained with insulin was linearly related to the number of plated cells. These results suggest that insulin stimulates erythroid colony formation by a direct action on CFU‐e.This publication has 16 references indexed in Scilit:
- Measurement of Somatomedin-Related Peptides in Fetal, Neonatal, and Maternal Rat Serum by Insulin-Like Growth Factor (IGF) I Radioimmunoassay, IGF-II Radioreceptor Assay (RRA), and Multiplication- Stimulating Activity RRA after Acid-Ethanol Extraction*Endocrinology, 1982
- Effects of insulin on cellular growth and proliferationLife Sciences, 1981
- Radioimmunological determination of insulinlike growth factors I and II in normal subjects and in patients with growth disorders and extrapancreatic tumor hypoglycemia.Journal of Clinical Investigation, 1981
- The mitogenic activity of insulin: An intrinsic property of the moleculeBiochemical and Biophysical Research Communications, 1980
- Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterolExperimental Cell Research, 1980
- Nonsuppressible insulin-like activity (NSILA) from human serum: Recent accomplishments and their physiologic implicationsMetabolism, 1978
- Primary structure of human insulin‐like growth factor IIFEBS Letters, 1978
- Growth Hormone: Species-Specific Stimulation of Erythropoiesis in VitroScience, 1977
- Erythroid colony formation in cultures of mouse and human bone marrow: Analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose‐concanavalin AJournal of Cellular Physiology, 1974
- Effects of Growth Hormone upon Erythropoiesis in the Hypophysectomized Rat.Experimental Biology and Medicine, 1954