Capping of the Lymphocyte C3 Receptor and Temperature-Dependent Loss of C3 Rosettes

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Abstract
The percentage of mouse spleen cells rosetted by bacteria-antibody-complement complexes (BAC) was found to be temperature dependent. Maximal rosetting was observed when BAC and spleen cells were incubated in ice (0.5°C), whereas less rosetting resulted from 37°C incubations. When rosetted cells were incubated at 37°C, cap formation was found to occur, and indirect evidence suggested that the capped bacteria were subsequently shed from the lymphocyte surface. Neither capping nor shedding occurred at 0.5°C, suggesting that these phenomena were responsible for the decreased percentage of rosetted cells found after incubation at 37°C. An incidental observation indicated that a second factor potentially contributing to the loss of rosetted cells at 37°C was the removal of BAC from the surface of cells by phagocytes.

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