DETECTION OF A SPECIES OF BOVINE THEILERIA AND ITS ANTIBODY BY FLUORESGENT ANTIBODY TECHNIQUE

Abstract
The fluorescent antibody technique was applied to the detection of bovine theileriosisexisting in Japan. A direct fluorescent antibody technique was useful for detectingcausative protozoa of the theileriosis, or so-called small-type piroplasrrtas. Antibodycould be detected by the indirect fluorescent antibody technique from cattle infectedwith small-type piroplasmas. Ill both experimentally and naturally infected cattle, serum antibody titer was almost parallel to the number of parasites in erythrocytes. Ahigh antibody titer, however, persisted after recovery from the disease. Nevertheless, parasites decreased gradually in nurrnber. The gross anatomy of the course auad distribution of the N. intestiuaalis in the fowlis described and illustrated in detail on the basis of 17 complete dissections. Especially.tlae origin of the N. intestinalis, its distribution to the duodenum, cecum and cloaca, itsrelation to the Plexus celiacus and mesentericus cranialis, and its anastomosis with theN. N73gUS are discussed.

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