An unusual route to thermostability disclosed by the comparison ofthermus thermophilusandescherichia coliinorganic pyrophosphatases

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Abstract
The structures ofEscherichia colisoluble inorganic pyrophosphatase (E‐PPase) andThermus thermophilussoluble inorganic pyrophosphatase (T‐PPase) have been compared to find the basis for the superior thermostability of T‐PPase. Both enzymes are D3hexamers and crystallize in the same space group with very similar cell dimensions. Two rather small changes occur in the T‐PPase monomer: a systematic removal of Ser residues and insertion of Arg residues, but only in the C‐terminal part of the protein, and more long‐range ion pairs from the C‐terminal helix to the rest of the molecule. Apart from the first five residues, the three‐dimensional structures of E‐PPase and T‐PPase monomers are very similar.The one striking difference, however, is in the oligomeric interactions. In comparison with an E‐PPase monomer, each T‐PPase monomer is skewed by about 1 Å in thexyplane, is 0.3 Å closer to the center of the hexamer in thezdirection, and is rotated by approximately 7° about its center of gravity. Consequently, there are a number of additional hydrogen bond and ionic interactions, many of which form an interlocking network that covers all of the oligomeric surfaces. The change can also be seen in local distortions of three small loops involved in the oligomeric interfaces. The complex rigid‐body motion has the effect that the hexamer is more tightly packed in T‐PPase: the amount of surface area buried upon oligomerization increases by 16%. The change is sufficiently large to account for all of the increased thermostability of T‐PPase over E‐PPase and further supports the idea that bacterial PPases, most active as hexamers or tetramers, achieve a large measure of their stabilization through oligomerization. Rigid‐body motions of entire monomers to produce tighter oligomers may be yet another way in which proteins can be made thermophilic.
Funding Information
  • Finnish Academy of Sciences
  • Suomen Tiedeakatemia
  • N1 ((1444 to A.G. and 3875 to R.L.))