Monkey interferon: Activity on human cells and chromatographic properties

Publisher Website
Google Scholar
Abstract
Monkey interferon (MKIF) produced in monkey BSC-1 cells infected with Newcastle disease virus showed antiviral activity on human foreskin fibroblasts and RD114 cells—a human line transformed by feline sarcoma virus. The titer of the monkey interferon in human cells was 10–30 fold greater than that found in several normal monkey (BSC-1, CV-1) or SV40 transformed (C2, C6, T-22) monkey cell lines tested. Ten to fifteen-fold purification of MKIF without loss of activity could be achieved by chromatography on Phenyl-Sepharose CL-4B. Antiviral activity of MKIF was fully resistant to treatment with 1 per cent sodium dodecyl sulfate (SDS).