Assembly Map of the 50‐S Subunit from Escherichia coli Ribosomes, Covering the Proteins Present in the First Reconstitution Intermediate Particle

Publisher Website
Google Scholar
Abstract
Highly purified proteins and 23-S RNA from the 50-S subunit of E. coli ribosomes were used to study the assembly dependences of the early assembly proteins. The proteins under observation and the RNA were incubated at 4 mM Mg2+ and 44.degree. C, the unbound proteins were separated by sucrose gradient centrifugation, the RNA.cntdot.protein complex was precipitated with trichloroacetic acid, and the complex-bound proteins were identified by means of sodium dodecyl sulfate gel electrophoresis. A systematic analysis led to the establishment of an assembly map including 17 proteins which represent the protein moiety of the 1st reconstitution intermediate particle.