Correcting errors in estimating neuron area caused by the position of the nucleolus

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Abstract
The technique of measuring soma cross‐sectional area at the plane of the nucleolus leads to systematic errors that depend on how far the nucleolus is displaced from the center of the soma. A set of correction factors was produced based on calculations from a geometric model of the measurement process. Applying the correction factors to measurements of second‐order auditory neurons led to substantial changes in the estimated soma area.