Nitric oxide synthase in cultured endocardial cells of the pig
Open Access
- 1 September 1991
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 104 (1) , 21-24
- https://doi.org/10.1111/j.1476-5381.1991.tb12378.x
Abstract
Endocardial cells release factors which regulate myocardial contractility and guanosine 3′:5′‐cyclic monophosphate (cyclic GMP) levels. One of these factors is indistinguishable from endothelium‐derived relaxing factor (EDRF). The effluent from pig heart endocardial cells cultured on microcarrier beads caused the relaxation of a pig coronary artery ring denuded of endothelium. This relaxation was enhanced by a combination of superoxide dismutase and catalase and was attenuated by haemoglobin, which binds nitric oxide (NO), and by inhibitors of NO synthase, NG‐monomethyl‐l‐arginine (l‐NMM A) or NG‐nitro‐l‐arginine. A Ca2+‐, l‐arginine‐ and NADPH‐dependent enzyme activity which generated NO was detected by a specific spectrophotometric assay in cytosol prepared from endocardial cells. The formation of NO was inhibited in a concentration‐dependent manner by l‐NMMA (but not d‐NMMA) and this could be partially reversed upon addition of excess l‐arginine. Like endothelial cells from the blood vessels, the endocardial cells possess the ability to synthesize NO, which may act to regulate myocardial contractility.Keywords
This publication has 40 references indexed in Scilit:
- Dexamethasone prevents the induction by endotoxin of a nitric oxide synthase and the associated effects on vascular tone: An insight into endotoxin shockPublished by Elsevier ,2005
- Induction of nitric oxide synthase by cytokines in vascular smooth muscle cellsFEBS Letters, 1990
- Anti-inflammatory glucocorticoids inhibit the induction by endotoxin of nitric oxide synthase in the lung, liver and aorta of the ratBiochemical and Biophysical Research Communications, 1990
- Nitric oxide from L-arginine stimulates the soluble guanylate cyclase in adrenal glandsBiochemical and Biophysical Research Communications, 1989
- Biosynthesis of endothelium-derived relaxing factor: A cytosolic enzyme in porcine aortic endothelial cells Ca2+-dependently converts L-arginine into an activator of soluble guanylyl cyclaseBiochemical and Biophysical Research Communications, 1989
- A novel citrulline-forming enzyme implicated in the formation of nitric oxide by vascular endothelial cellsBiochemical and Biophysical Research Communications, 1989
- Nitric oxide: A cytotoxic activated macrophage effector moleculeBiochemical and Biophysical Research Communications, 1988
- L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxationBiochemical and Biophysical Research Communications, 1988
- Heart involvement in metastatic carcinoid diseaseClinical Cardiology, 1986
- The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 1980