Immunocytochemical and enzyme-cytochemical localization of intestinal alkaline phosphatase of rats.

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Abstract
Two different techniques, enzyme-cytochemical and immunocytochemical, were employed to localize alkaline phosphatase in the rat small intestine at light microscopic and EM levels. In the absorptive cells of the duodenum and jejunum, alkaline phosphatase was localized by the classical enzyme-cytochemical technique on the surfaces of apical microvilli and lateral membranes; the direct immunoperoxidase method revealed some additional sites of the enzyme, such as cisternae of rough endoplasmic reticulum and perinuclear spaces. Both enzyme- and immunocytochemistry failed to demonstrate the enzyme in the absorptive cells of the ileum. The absorptive cells in the proximal small intestine synthesize and transport the enzyme protein to their cell surfaces.

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