Concanavalin-A-induced transmembrane linkage of concanavalin A surface receptors to intracellular myosin-containing filaments.

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Abstract
The distribution on the cell surface of receptors for concanavalin A Con A, and the distribution of the smooth muscle myosin-like protein inside the same cell, was studied using specific fluorescence microscopic methods with normal rat kidney cells in monolayer culture. The Con A receptors were initially uniformly dispersed over the cell surface, but 20 min after the addition of Con A at 37.degree. the receptors showed a variety of nonuniform surface distributions, including extended parallel linear arrays. These arrays of receptors were superimposed on the linear arrays of the intracellular myosin-containing filaments, indicating that a transmembrane linkage of the receptors and the filaments had occurred. This linkage required a lateral redistribution of Con A receptors, since it did not occur with succinylated Con A but was subsequently induced if the cells that had been reacted with succinylated Con A were then treated with antibodies to Con A. The redistributions of Con A receptors on the surfaces of these normal rat kidney cells were much less extensive than the patching that was induced on the surfaces of the same cells infected with, and transformed by, Rous sarcoma virus.

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