Differentiation of Gram Positive and Gram Negative Bacteria in Transparent Acrylic Resin Emulsion Replicas of Surfaces of Plants

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Abstract
When acrylic resin emulsion (Rohm & Haas Primal AC‐33) is allowed to dry on a leaf surface, it can be peeled off to give a transparent replica of the surface with bacteria and fungi embedded in it. The distribution of micro‐organisms in the replica appears to reflect the patterns in which they occur naturally on the surface. The resin replicas may be stained by a variety of microscopical stains, the best of which is phenol‐acetic‐aniline blue, and are suitable for high power light microscopy.Acrylic resin emulsion differs from widely used cellulose‐based materials in that permanent differentiated preparations of Gram positive and Gram negative stained bacteria may be produced from a wide variety of types of surface.