High-affinity arabinose transport mutants of Escherichia coli: isolation and gene location

Publisher Website
Google Scholar
Abstract
The gene araF, the product of which is the L-arabinose-binding protein (a component of the high-affinity L-arabinose transport system), was located on the E. coli linkage map at 45 min. This location was established using bacteriophage P2 eductates and bacteriophage P1 cotransduction frequencies with the adjacent genetic loci, his (histidine biosynthesis) and mgl (methylgalactoside transport). Mutants that phenotypically exhibited altered high-affinity L-arabinose transport capacities were also isolated. At least 2 of these mutations were located in the araF gene, as binding protein purified from these strains exhibited altered in vitro arabinose-binding properties.

This publication has 31 references indexed in Scilit: