Use of inter-proton nuclear Overhauser effects to assign the nuclear magnetic resonance spectra of oligodeoxynucleotide and hybrid duplexes in aqueous solution

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Abstract
Inter‐proton nuclear Overhauser enhancements (NOEs) have been used to assign the aromatic, anomeric and 2′ resonances in the 1H nuclear magnetic resonance spectrum of the duplex formed between d(T‐C‐A‐C‐A‐T) and d(A‐T‐G‐T‐G‐A). The same techniques have been applied to assignments in the hybrid duplex formed by d(T‐C‐A‐C‐A‐T) with r(A‐U‐G‐U‐G‐A). Comparison of intra‐residue with inter‐residue NOEs yields structural information which suggests that the conformations of both duplexes are similar. The NOEs are consistent with inter‐proton distances measured from models of B‐form DNA. Circular dichroic results confirm these deductions.