Isolation of Mycoplasma from Human Root Canals

Abstract
Cultural and morphological characteristics of Mycoplasma isolated from human root canals were investigated. These organisms, tentatively identified as Mycoplasma salivarium, lost their ability to form colonies when placed in brewer thioglycolate medium, glucose ascites broth, and trypticase soy broth. Chloramphenicol and kanamycin, at concentrations of 0.1 [mu]g./ml., inhibited growth of strains RC1 and RC2. Storage at -60[degree]C. provided maximum preservation of colony forming ability of strain RC1.