Induction of zebrafish (Brachydanio rerio) P450in vivoand in cell culture
- 1 January 1994
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 24 (6) , 487-493
- https://doi.org/10.3109/00498259409043251
Abstract
1. Induction of zebrafish P450 by 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) was studied in liver tissue, primary liver cell culture and multipassage cell culture derived from zebrafish haploid and diploid embryos and liver. 2. TCDD induced two hepatic proteins (54 and 50 kDa) in vivo which were recognized by anti-trout P4501A1 IgG. The 54-kDa protein was induced by TCDD in primary and multipassage hepatocyte cultures and in haploid and diploid embryo-derived cells. The proteins in liver homogenates were not induced by aqueous exposure of zebrafish to β-naphthoflavone (BNF). 3. Homogenates of zebrafish liver, cultured hepatocytes and embryo-derived cells also exhibited increased ethoxyresorufin O-deethylase (EROD) and 7-12-dimethyl-benz[a]anthracene (DMBA) hydroxylase activity following TCDD exposure.Keywords
This publication has 24 references indexed in Scilit:
- Hepatic CYP1A1 Induction in Rainbow Trout by Continuous Flowthrough Exposure to β-NaphthoflavoneFundamental and Applied Toxicology, 1993
- Regulation of the cytochrome P450 enzyme system in fishAquatic Toxicology, 1992
- Toxicity of mercury, copper, nickel, lead, and cobalt to embryos and larvae of zebrafish,Brachydanio rerioArchives of Environmental Contamination and Toxicology, 1991
- Early life-stage test in zebrafish versus a growth test in rainbow trout to evaluate toxic effectsBulletin of Environmental Contamination and Toxicology, 1991
- Immunochemical cross-reactivity ofβ-naphthoflavone-inducible cytochrome P450 (P450IA) in liver microsomes from different fish species and ratFish Physiology and Biochemistry, 1991
- Cytochrome P-450 Monooxygenase Systems in Aquatic Species: Carcinogen Metabolism and Biomarkers for Carcinogen and Pollutant ExposureEnvironmental Health Perspectives, 1991
- Characterization and induction of xenobiotic metabolizing enzyme activities in a primary culture of rainbow trout hepatocytesXenobiotica, 1991
- Detection and characterization of 63H92,3,7,8-tetrachlorodibenzo-p-dioxin binding to Ah receptor in a rainbow trout hepatoma cell line*1Toxicology and Applied Pharmacology, 1990
- A long-term toxicity test comprising reproduction and growth of zebrafish with 4-chloroaniiineArchives of Environmental Contamination and Toxicology, 1990
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981