Contribution à l’étude immunochimique des hémolysats de globules rouges humains

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Abstract
Some minor components of total hemolysates of human red blood cells were concentrated after elimination of the hemoglobin by treatment with ethanol and chloroform. Butanol was used for the solubilization of different fractions of the erythrocytic stromata. Several components of the hemolysate were characterized after electrophoresis in agar, immunoelectrophoretic analysis and double diffusion in agar, combined with enzymatic methods different hemoglobin fractions, catalase, 4 carboxylic acid esterases, and 1 protease. Several hemopathies showed abnormal immunologic patterns: thalassemia minor and major, acatalasemia. The immunoelectrophoretic analysis of the hemolysate actually gave 12 lines of which 3 only were identified.