ESTABLISHMENT OF CELL LINE DERIVED FROM HUMAN MALIGNANT-MELANOMA

  • 1 January 1978
    • journal article
    • research article
    • Vol. 25  (6) , 701-712
Abstract
A new human cell line of malignant melanoma (MJM) was established with the use of the in vitro fragment technique. It has been maintained over 34 mo. of continuous cultivation. Three types of cells can be recognized by light microscopy. The epithelioid elements predominate, less frequent are fibroblastoid and giant multinuclear cells. The pigment production is not macroscopically visible. Over 60% of analyzed metaphases showed hyperdiploid number of chromosomes, the rest was mostly tetra and hexaploid. No marker chromosomes were detected. Growth studies indicate that MJM cells have 63 h doubling time. Cytochemistry revealed positive pigment or propigment granules in 36% of cells. Ultrastructural studies did not detect melanin granules but some particles resembling atypical premelanosomes and melanosomes were recognized in some sections.

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