The Optimization of Density Gradients for Zonal Centrifugation
- 1 December 1969
- journal article
- research article
- Published by Taylor & Francis in Separation Science
- Vol. 4 (6) , 467-481
- https://doi.org/10.1080/01496396908052274
Abstract
By using established equations and techniques, it is possible to compute sucrose gradients which are optimized to minimize volume expansion of a zone during sedimentation in zonal rotors. These gradients have been tested experimentally, and it has been found that the greatest expansion of the sample zone occurred during the early stage of sedimentation. Following this expansion, the gradient maintained the volume within the limits set by the calculations.Keywords
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