Direct observation of kinesin stepping by optical trapping interferometry
- 1 October 1993
- journal article
- Published by Springer Nature in Nature
- Vol. 365 (6448) , 721-727
- https://doi.org/10.1038/365721a0
Abstract
Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.Keywords
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