ANTIGENS OF PASTEURELLA MULTOCIDA TYPE I .2. LIPOPOLYSACCHARIDES

Google Scholar
  • 1 January 1961
    • journal article
    • research article
    • Vol. 4  (2) , 122-+
Abstract
Asian strains of Pasteurelia multocida Type I were extracted with saline to remove superficial material and then with phenol-water to extract the lipopolysaccharides. This product was recovered from the aqueous phase by centrifuging at 105,000 g for 2 hours. The component sugars are galactose, glucose and glucosamine and a heptose sugar, possibly D-glycero-L-manno-heptose. The preparation was toxic for rabbits and was absorbed on human erythrocytes. An Australian strain of P. multocida, which by conventional mouse protection and haemagglutination tests was identified as Type I yielded a lipopolysaccharide with different properties. The disease of cattle, haemorrhagic septicaemia, which is caused by P. multocida Type I in Asia is unknown in Australia.