Screening of basidiomycetes for lignin peroxidase genes using a DNA probe
- 1 January 1990
- journal article
- research article
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 32 (4) , 436-442
- https://doi.org/10.1007/bf00903779
Abstract
Basidiomycetes were screened for lignin peroxidase (LPO) genes using a DNA probe prepared from the LPO restriction fragment ofPhanerochaete chrysosporium. Southern blot analysis showed restriction fragments of chromosomal DNA ofBjerkandera adusta andCoriolus consors hybridized with the probe.Bjerkandera adusta produced LPO in a glucose-peptone medium. Ion-exchange chromatography showed that this fungus produced multiple molecular forms of LPO. One of the enzymes, LPO-2, was purified and characterized. The molecular weight of LPO-2 was 41000 with a pI of 4.2. Spectral analysis demonstrated that LPO-2 is a haem protein. The enzyme cleaved lignin model dimers mainly at the Cα-Cβ position of the side chain. The LPO-2 exhibited close similarity to LPOs ofP. chrysosporium with respect to their basic properties.This publication has 31 references indexed in Scilit:
- Extracellular Peroxidases Involved in Lignin Degradation by the White Rot Basidiomycete Phanerochaete chrysosporiumPublished by American Chemical Society (ACS) ,1989
- Analysis of nucleotide sequences of two ligninase cDNAs from a white-rot filamentous fungus, Phanerochaete chrysosporiumGene, 1987
- Enzymatic "Combustion": The Microbial Degradation of LigninAnnual Review of Microbiology, 1987
- Cloning and sequencing of a cDNA for a ligninase from Phanerochaete chrysosporiumNature, 1987
- Production of multiple ligninases by Phanerochaete chrysosporium: effect of selected growth conditions and use of a mutant strainEnzyme and Microbial Technology, 1986
- Isolation and characterization of the Aspergillus niger trpC geneGene, 1985
- Ligninolytic enzyme production byPhanerochaete chrysosporiumunder conditions of nitrogen sufficiencyFEMS Microbiology Letters, 1984
- Separation and characterization of two extracelluar H2O2‐dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporiumFEBS Letters, 1984
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976