Probing Combinatorial Library Diversity by Mass Spectrometry
- 1 August 1997
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 69 (15) , 2893-2900
- https://doi.org/10.1021/ac970049w
Abstract
The feasibility of a “massively parallel” mass spectrometric method for probing combinatorial library diversity is addressed theoretically for the example of computer-generated mass distributions of combinatorially synthesized peptide libraries containing between two and seven amino acids. We study the behavior of several “global” (integral) parameters of such mass distributionsmass centroid, dispersion, skewness, and kurtosis. The centroid and dispersion are shown to carry information that may characterize the completeness of the synthetic effort. “Local” mass distribution parameters, e.g., “mass density” (number of peptides per mass interval), are also examined. The practical implementation and eventual limitations of such an approach are discussed as well.Keywords
This publication has 26 references indexed in Scilit:
- Novel Procedure for Molecular Weight Averages Measurement of Polydisperse Polymers Directly from Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass SpectraRapid Communications in Mass Spectrometry, 1996
- Accuracy Requirements for Peptide Characterization by Monoisotopic Molecular Mass MeasurementsAnalytical Chemistry, 1996
- Molecular Diversity and Libraries of Structures: Synthesis and ScreeningCollection of Czechoslovak Chemical Communications, 1996
- Evaluation of Mass Spectrometric Methods Applicable to the Direct Analysis of Non-Peptide Bead-Bound Combinatorial LibrariesAnalytical Chemistry, 1996
- Approaches and Limits for Accurate Mass Characterization of Large BiomoleculesAnalytical Chemistry, 1995
- Characterization of the Complexity of Small-Molecule Libraries by Electrospray Ionization Mass SpectrometryAnalytical Chemistry, 1995
- Bio‐affinity characterization mass spectrometryRapid Communications in Mass Spectrometry, 1995
- Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence TagsAnalytical Chemistry, 1994
- Applications of Combinatorial Technologies to Drug Discovery. 2. Combinatorial Organic Synthesis, Library Screening Strategies, and Future DirectionsJournal of Medicinal Chemistry, 1994
- Isotopic distributions in mass spectra of large moleculesAnalytical Chemistry, 1983