A method for micro-scale isolation of sulfated glycopeptide from tissue.

1 January 1979

journal article
research article
Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine

Vol. 129 (3) , 225-231
https://doi.org/10.1620/tjem.129.225

Abstract

Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of the following steps: pronase digestion, cetylpyridinium chloride-fractionation, crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment and preparative cellulose acetate membrane electrophoresis. By this method, sulfated glycopeptide was obtained in a high yield from estrogen-treated rabbit uterus.