A SEARCH FOR PARENTAL ANTIGENS NOT EXPRESSED IN F1 HYBRID MICE

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Abstract
Parental strain mice were given injections of F1 hybrid spleen cells at birth to induce tolerance. When these mice were at least 3 months old, they were tested by means of skin grafts and/or spleen or marrow cell suspensions for tolerance to antigens of the second parental type. Very long lasting tolerance to second parental skin was recorded for two inbred strains, but at best only partial tolerance was achieved in another two. Evidence of tolerance of some parental mice to spleen cell suspensions was obtained from wasting disease that resulted. In addition, positive identification was made of donor-type cells from the peritoneal cavities of some tolerant mice at 2–4 weeks after they had received spleen cell injections. Presumably-tolerant mice usually failed to produce circulating hemagglutinating or cytotoxic antibodies even when repeatedly immunized. This unresponsiveness was specific for second parental and F1 cells. These findings confirm and extend earlier work showing F1 cells capable of inducing tolerance to all antigens of one or both parental types. No evidence was obtained that there are parental antigens in the 4 inbred strains investigated that are not expressed in their respective F1 hybrids.

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