"No-touch" Technique using Saphenous Vein Harvested with its Surrounding Tissue for Coronary Artery Bypass Grafting Maintains an Intact Endothelium

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Abstract
Spasm and consequent dilation of the saphenous vein (SV) for coronary artery bypass grafting (CABG) can be avoided if the vein is harvested with its surrounding tissue. Morphologic techniques, including scanning and transmission electron microscopy, were used to compare endothelial cell integrity using three SV harvesting procedures: conventional (adventitial stripping of the vein, manual distention and storing in saline); intermediate (after adventitial stripping, the vein was left in situ, covered with a papaverine-soaked compress, and stored in heparinized blood); and ?no-touch? (SV dissected with its surrounding tissue was left in situ, covered with a saline-soaked compress and stored in heparinized blood). Preservation of endothelial cell integrity was greater with the ?no-touch? procedure than with the other methods. Since endothelial cell integrity of SV grafts may affect the patency rate, we conclude that the ?no-touch? preparation should improve the results of CABG.

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