In vitro plant regeneration of leguminous trees (Albizia spp)
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (6) , 385-388
- https://doi.org/10.1007/bf00269518
Abstract
Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardiana (1.6). Higher concentrations of auxins in the medium, in general, enhanced rooting and callusing but cytokinins promoted the growth of green calli. BA enchanced the differentiation of shoots in the three species. The in vitro grown shoots of A. amara and A. richardiana, after subculturing on B5+1 μM IAA developed roots (up to 30–40%). These plants have been successfully transferred to the field.Keywords
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