DNA Alkylation Damage as a Sensor of Nitrosative Stress in Mycobacterium tuberculosis
Open Access
- 1 February 2003
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 71 (2) , 997-1000
- https://doi.org/10.1128/iai.71.2.997-1000.2003
Abstract
One of the cellular consequences of nitrosative stress is alkylation damage to DNA. To assess whether nitrosative stress is registered on the genome of Mycobacterium tuberculosis, mutants lacking an alkylation damage repair and reversal operon were constructed. Although hypersensitive to the genotoxic effects of N-methyl-N′-nitro-N-nitrosoguanidine in vitro, the mutants displayed no phenotype in vivo, suggesting that permeation of nitrosative stress to the level of cytotoxic DNA damage is restricted.Keywords
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