IMMUNOBIOLOGICAL PROPERTIES OF 1-BUTANOL-EXTRACTED CELL-SURFACE ANTIGENS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (10) , 3956-3960
Abstract
Extracts of viable 3-methylcholanthrene-induced murine sarcoma cells (MCA-F and MCA-2A) prepared using single-phase (2.5%) 1-butanol significantly retarded the outgrowth of the homotypic but not the heterotypic tumor of syngeneic C3H/HeJ mice. Butanol extracts specifically evoked a delayed hypersensitivity response in tumor-immune syngeneic mice, but not in alloimmune DBA/2J mice. Crude butanol extracts of MCA-F cells did not contain alloantigenic activity, as shown by their inability to block H-2 or Ia-specific antibodies in a complement-dependent cytotoxicity assay. Absorption of these same allospecific reagents with untreated or with butanol-extracted cells indicated that H-2 antigens remain associated with the cell surface during extraction. Butanol appears to release tumor antigens but not alloantigens from the cell surface.