Intramembranous ossification mechanism for bone bridge formation at the growth plate cartilage injury site
- 1 March 2004
- journal article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 22 (2) , 417-426
- https://doi.org/10.1016/j.orthres.2003.08.003
Abstract
Salter's type III and type IV growth plate injuries often induce bone bridge formation at the injury site. To understand the cellular mechanisms, this study characterized proximal tibial transphyseal injury in rats. Histologically, bony bridge trabeculae appeared on day 7, increased on day 10, and became well-constructed on day 14 with marrow. Prior to and during bone bridging, there was no cartilage proteoglycan metachromatic staining and no collagen-X immunostaining at the injury site, nor was there any up-regulation of BrdU-labelled chondrocyte proliferation at the adjacent physeal cartilage, suggesting no new cartilage formation at the injury site. However, infiltration of vimentin-immunopositive mesenchymal cells from metaphysis and epiphysis was apparent on day 3, with the mesenchymal population being prominent on days 7 and 10 and subsided on day 14. Among these infiltrates were osteoprogenitor precursors expressing osteoblast differentiation factor (cbf-alpha1) on day 3, along with some cbf-alpha1+ osteoblast-like cells lining bone trabeculae on days 7 and 10. Some mesenchymal cells and trabecula-lining cells were also alkaline phosphatase-immunopositive, further suggesting their osteoblast differentiation. From day 7 onwards, some trabecula-lining cells became osteocalcin-producing mature osteoblasts. These results suggest that bone bridge formation after growth plate injury occurs directly via intramembranous ossification through recruitment of marrow-derived osteoprogenitor cells.Keywords
This publication has 20 references indexed in Scilit:
- Utilization of a Murine Model to Investigate the Molecular Process of Transphyseal Bone FormationJournal of Pediatric Orthopaedics, 2000
- Utilization of a Murine Model to Investigate the Molecular Process of Transphyseal Bone FormationJournal of Pediatric Orthopaedics, 2000
- Regulation of Osteoblast Differentiation Mediated by Bone Morphogenetic Proteins, Hedgehogs, and Cbfa1Endocrine Reviews, 2000
- Does adult fracture repair recapitulate embryonic skeletal formation?Mechanisms of Development, 1999
- Type X collagen is colocalized with a proteoglycan epitope to form distinct morphological structures in bovine growth cartilageBone, 1996
- Epiphyseal Distraction and Centrally Located Bone Bar: An Experimental Study in the RabbitJournal of Pediatric Orthopaedics, 1996
- Growth-Plate Modifications After DrillingJournal of Pediatric Orthopaedics, 1994
- The Demonstration of Bone and Cartilage Remodelling Using Alcian Blue and HematoxylinStain Technology, 1988
- Bone development and repairBioEssays, 1987
- Evolution of the Concept of an Extensible Nail Accommodating to Normal Longitudinal Bone GrowthClinical Orthopaedics and Related Research, 1981