Radioimmunoassay of Testosterone-Estradiol-Binding Globulin in Humans: A Reassessment of Normal Values*

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Abstract
Antiserum against human testosterone-estradiolbinding globulin (hTeBG) was prepared in rabbits, and its specificity was demonstrated by crossed immunoelectrophoresis. A RIA for the measurement of hTeBG in serum was developed. With this assay, hTeBG was readily measured in 1–2 μ serum. Intraassay coefficients of variation for pools of serum from men, women, and women in late pregnancy were 7.7%, 5.5%, and 6.1%, respectively. Interassay coefficients of variation for the same pools were 10%, 12%, and 12%, respectively. The TeBG levels in a number of nonselected subjects determined by the present method show good correlations with those obtained by steady state polyacrylamide gel electrophoresis and dextran-coated charcoal assay. The concentrations of TeBG determined by the RIA in sera from men, women, and women in late pregnancy were 18 ± 9 (n = 12), 54 ± 13 (n = 8), and 374 ± 55 (n = 6) pmol/ ml (mean ± SD), respectively. (J. Clin Endocrinol Metab56: 68,1983)