The detection of superoxide anion from the reaction of oxyhemoglobin and phenylhydrazine using EPR spectroscopy
- 1 April 1979
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 35 (4) , 488-489
- https://doi.org/10.1007/bf01922723
Abstract
The low temperature EPR spectrum of a quickly reacted mixture of oxyhemoglobin and phenylhydrazine was studied. With the use of a computer, the spectral contribution of methemoglobin in the region of g=2 was subtracted. The remaining spectrum was that of an axial free radical (g⊥=2.00, g‖=2.06) having the magnetic parameters of superoxide anion. In the presence of superoxide dismutase, this axial radical was not seen, confirming that superoxide anion is indeed generated by the reaction.Keywords
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