Detection of Mycoplasmalike Organisms in Infected Blueberry Cultivars by the DAPI Technique

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Abstract
Coded samples from stunt-infected and healthy blueberry plants were evaluated for the presence of mycoplasmalike organisms (MLO) by comparing 2 histological techniques. By the DAPI (4'',6-diamidino-2-phenylindole) technique with root tissue, MLO were directly detected; by the aniline blue (AB) technique with petiole samples, MLO were indirectly monitored by detecting the resulting excess callose in sieve tubes. With DAPI, 24 of 25 infected and 25 or 25 healthy root samples were correctly evaluated. With AB, 19 of 25 infected and 10 of 25 healthy petiole samples were correctly evaluated. MLO were observed by EM in root samples from 1 stunt-infected, DAPI-positive plant but not in root samples from 1 known healthy, DAPI-negative blueberry plant. Root samples from clones of several symptomless highbush blueberry cultivars were classified as MLO-positive by the DAPI techique. Petiole and stem samples from these sample plants were rated negative by the DAPI test.

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