Interferon-γ-induced JAK2 and STAT1 signalling in a human salivary gland cell line

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Abstract
We have used a human salivary gland cell line (HSG) as a possible in vitro model to evaluate the effects of IFN-γ on human salivary gland epithelium (Wu et al., 1994, 1996, 1997). In the present study, we examined the JAK-STAT signal-transduction pathway in IFN-γ-treated HSG cells. We demonstrate that JAK2 and Stat1 are phosphorylated at tyrosine residues in a time- and concentration-dependent manner following exposure to IFN-γ. In addition, we show that activation of this signalling pathway is decreased by the addition of a blocking antibody to the IFN-γ receptor. The same maneuver is also able to reduce by approximately 50–70% the surface expression of two IFN-γ-induced immunoregulatory molecules: HLA-DR and ICAM-1. These results demonstrate that the JAK2 and Stat1 signalling pathway is active in salivary-derived epithelial cells and may contribute to their immunopathologic destruction. J. Cell. Physiol. 173:110–114, 1997. © 1997 Wiley-Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.
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