SOME OBSERVATIONS ON THE „LATENT” RIBONUCLEASE OF ESCHERICHIA COLI
- 1 June 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 51 (6) , 1267-1274
- https://doi.org/10.1073/pnas.51.6.1267
Abstract
Most of the so-called "latent" ribonuclease of E. coli can be released from spheroplasts in 5 min. although the spheroplast ribosomes remain intact as demonstrated by RNA content, sucrose-density gradient profiles and ability to adsorb soluble RNase. Ribosomes adsorb up to a 12-fold excess of soluble RNase even under dissociated conditions. All of the RNase binds to 30s particles. Spheroplasts prepared by lysozyme alone release neither alkaline phosphatase nor RNase, but nearly half of the alkaline phos-phatase can be removed by washing EDTA-treated E. coli, although other enzymes are not released.Keywords
This publication has 11 references indexed in Scilit:
- On the cytological unit for protein synthesis in vivo in E. coliBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964
- The release of ribonuclease into the medium when E.coli cells are converted to spheroplastsBiochemical and Biophysical Research Communications, 1964
- Protein synthesis by polyribosomes on protoplast membranes of B. megateriumJournal of Molecular Biology, 1963
- Beakdown of messenger ribonucleic acid by a potassium-activated phosphodiesterase from Escherichia coli.1963
- Resistance of S-RNA to ribonucleases in the presence of magnesium ionBiochemical and Biophysical Research Communications, 1963
- The reversible release of deoxyribonuclease, protein and ribonucleic acid from ribosomesBiochimica et Biophysica Acta, 1963
- The location of ribonuclease in Escherichia coli☆Biochimica et Biophysica Acta, 1963
- The localization of alkaline phosphatase in E., coli K12Biochemical and Biophysical Research Communications, 1961
- Latent enzymic activity of a ribonucleoprotein isolated from Escherichia coliBiochimica et Biophysica Acta, 1959
- Lysis of gram-negative organisms and the role of verseneBiochimica et Biophysica Acta, 1958