Interactions of primary neuroepithelial progenitor and brain endothelial cells: distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact
- 26 June 2007
- journal article
- Published by Springer Nature in Cell Research
- Vol. 17 (7) , 619-626
- https://doi.org/10.1038/cr.2007.53
Abstract
Neurovascular interactions are crucial for the normal development of the central nervous system. To study such interactions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelial cell fractions from embryonic mouse brains. Depending on the culture conditions endothelial cells were found to favor maintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differentiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellular interactions needed for the proper development of the brain.Keywords
This publication has 17 references indexed in Scilit:
- Neural guidance molecules regulate vascular remodeling and vessel navigationGenes & Development, 2005
- Role of neural guidance signals in blood vessel navigationCardiovascular Research, 2005
- Guidance of vascular and neural network formationCurrent Opinion in Neurobiology, 2005
- The neurovascular unit and its growth factors: coordinated response in the vascular and nervous systemsNeurological Research, 2004
- VEGF: once regarded as a specific angiogenic factor, now implicated in neuroprotectionBioEssays, 2004
- Endothelial Cells Stimulate Self-Renewal and Expand Neurogenesis of Neural Stem CellsScience, 2004
- For the Long RunNeuron, 2004
- Impaired brain angiogenesis and neuronal apoptosis induced by conditional homozygous inactivation of vascular endothelial growth factorThrombosis and Haemostasis, 2004
- CULTURE OF MURINE BRAIN MICROVASCULAR ENDOTHELIAL CELLS THAT MAINTAIN EXPRESSION AND CYTOSKELETAL ASSOCIATION OF TIGHT JUNCTION–ASSOCIATED PROTEINSIn Vitro Cellular & Developmental Biology – Animal, 2003
- Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.The Journal of cell biology, 1984